Campaigners stopped traffic in east London as they took part in a parade down Whitechapel High Street.
The march was organised by friends of The Cass Faculty of Arts to highlight the need for creative education in Tower Hamlets.
Last year students staged protests after London Metropolitan University announced plans to sell Central House where The Cass is based.
The University has now finalised the sale of the building to a developer and will move its operations to one campus in north London.
But students and supporters of The Cass say it will lead to staff redunduncies and will leave thousands in east London without access to art courses.
A spokesperson for London Metropolitan University said, "We are passionate about keeping arts education in London, and making it available to people from all walks of life.
That's why we're investing £125m to create a new home for our Sir John Cass Faculty of Art, Architecture and Design at our flagship campus in Islington."